FLOT1 and neuroblastoma: As CHAPSO gradients yielded the highest distribution of the raft marker flotillin-1 and cholesterol in the low density fractions and Triton yielded the lowest distribution of flotillin-1 and cholesterol in the low-density fractions we next investigated the distribution of this marker in other primary hippocampal cultures and the neuroblastoma cell line SHSY5Y (Figure 4).