The differences in cellular response to T between the embryonal carcinoma cells and GC-1 cell used here may be due to differences in sensitivity to inhibition of KDM1 by T. Unlike GC-1 cells, embryonal carcinoma cells express high levels of KDM1 and have detectable levels of Pou5f1 under control conditions [43]. Here, POU5F1 is linked to embryonal carcinoma.