4). The results indicated that Hyal-1 was overexpressed in cancerous samples compared to macroscopically normal ones. Semi-quantitative analysis of the results revealed increased Hyal-1 expression especially in B2, C1 and C2 stage of cancer (fig. 4A). PH-20 was overexpressed at early stages of cancer, especially in the cancerous samples; considerable expression was also observed in benign tumors (fig. 4B). This evidence concerns the gene SPAM1 and cancer.