Since there were high levels of glucose (4.5g/dL) in both control and lactic acidosis media, we further clarified the role of glucose vs. lactic acidosis in the TXNIP induction using real-time PCR to determine the level of TXNIP in MCF-7 cultured under different glucose and lactic acidosis conditions (Figure 3D). The gene discussed is TXNIP; the disease is lactic acidosis.