Therefore, if release of S100B is due to a pathology initiating active synthesis rather than by passive release from necrotic astroglial cells (as in stroke) quantitative RT-PCR of S100B messenger RNA would be appropriate to further support any observations; 2) Antibody-independent methods such as MS should be included for validation of target recognition; 3) When possible HPLC (high-performance liquid chromatography) should be used in parallel or serial experiments. The gene discussed is S100B; the disease is stroke disorder.