Analysis of the number of ookinetes in the midgut lumen that were exhibiting condensed chromatin in the presence of a NOS inhibitor or the inactive isomer indicated significant differences due to treatments, (F2,12 = 123.86, P<0.001), time post-infection, (F1,12 = 63.379, P<0.001), and treatments by time interaction (F2,12 = 7.05796, P<0.01). The gene discussed is NOS2; the disease is infection.