GAPDH and cancer: However, the quantity of amplified FATS DNA fragment was significantly less in comparison with that of GAPDH DNA control in all these cancer cells (Figure 4A), indicating that the loss of mRNA expression of FATS is not only due to genomic deletion of coding strand in cancer genome, which is supported by loss of heterozygosity (LOH) studies [12,13], but also due to inhibition of gene transcription.