We were able to confirm by qRT-PCR that phospholipase A2 group 4c (Pla2g4c), gasdermin c2 (Gsdmc2 also known as MLZE, melanoma-derived leucine zipper-containing extranuclear factor), and mast cell protease 2 (Mcpt2) (Table 1), were all upregulated in CF controls and downregulated to WT control levels in lubiprostone treated CF mice (Fig. 7C-E). Here, PLA2G4C is linked to cystic fibrosis.