To investigate the expression levels of CENP-F, real-time RT-PCR, and western blotting analysis were conducted on the samples of normal nasopharyngeal epithelial cells (NPEC2), the immortalized nasopharyngeal epithelial cells (NPEC2 Bmi-1), and various NPC cell lines. The gene discussed is BMI1; the disease is nasopharyngeal carcinoma.