In this context, here, we have thoroughly studied the effects of Hsp90 inhibition by 17-AAG on the Hsp90-assisted signaling repertoire associated with cell cycle progression, apoptosis and motility in three human urinary bladder cancer cell lines of different malignancy grade, namely RT4 (grade I), RT112 (grade I-II) and T24 (grade III). This evidence concerns the gene HSP90AA1 and urinary bladder cancer.