Conditioned medium from MLO-Y4 cells and WiDr human colon carcinoma cells, which express large amounts of PLN, were added in triplicate (three samples from the same plate) to each of two blots and probed with an anti-PLN Ab (mouse anti-PLN, clone A76) or with secondary Ab only as a negative control. The gene discussed is PLN; the disease is colon carcinoma.