Following TB10.4 stimulation, analysis of IFN-γ alone resulted in 76% correct classification into TB cases or TST+ contacts while analysis of TNF-α, IL-12(p40) and IL-17 in combination increased this to 85% mainly due to an increase in specificity from 44% to 61% with sensitivity increasing from 92% to 97%. The gene discussed is TNF; the disease is tuberculosis.