Our results showed that Twist expresse in human BT tissues was significantly correlated to the tumor stage, grade and progression (P = 0.000, P = 0.000 and P = 0.021, respectively);Snail was significantly correlated to nodal involvement (P = 0.000), but Snail was not involved in bladder tumor differentiation, stage, grade and progression;Slug was only significantly correlated to nodal involvement (P = 0.012). The gene discussed is TWIST1; the disease is neoplasm.