However, it has been demonstrated in human tumor cell lines that the forced expression of E-cadherin was not sufficient to reverse the process in Slug, Snail or Twist-expressing cells, and this result supports that Slug, Snail or Twist might modulate other important signaling pathways involved in tumor progression and metastasis development independently of E-cadherin expression[38-42]. This evidence concerns the gene TWIST1 and neoplasm.