The expression of Snail, Slug, Twist and E-cadherin was analyzed at the mRNA and protein level by semiquantitative RT-PCR(Fig. 1A) and western blot (Fig. 1B) in the human bladder cancer cell lines T24, HTB-3, HTB-1, HTB-2 and HTB-9. Here, TWIST1 is linked to urinary bladder carcinoma.