There was a significant correlation between Twist overexpression and the tumor stage (P = 0.000)and grade(P = 0.000): superficial BT (Ta-1) (19 out of 76: 25%) and invasive BT (≥T2) (34 out of 44: 77.27%), LG (8 out of 41:19.51%), and HG (45 out of 79: 56.96%). Here, TWIST1 is linked to neoplasm.