IMPDH2 and osteosarcoma: To verify this hypothesis we established osteosarcoma cell lines with modulated IMPDH2 expression either by overexpression of the IMPDH2 coding sequence in Saos-2 cells (Saos-2 cdsIMPDH2) or by IMPDH2 knock-down using an shRNA construct specific for IMPDH2 (Saos-2 shIMPDH2).