Pathway Studio 7.0 was used to find interactions among the 12 genes and 13 major lung cancer hallmarks (EGF, EGFR, KRAS, MET, RB1, TP53, E2F1, E2F2, E2F3, E2F4, E2F5, AKT1, and TNF) reported in the literature. The gene discussed is RB1; the disease is lung cancer.