To test this hypothesis we over-expressed SRF or TFAP2 in SH SY5Y cells, HEK293 cells, and in lymphoblasts obtained from an Friedreich ataxia patient or from a healthy control individual, and qRT-PCR was performed to assess changes in cellular frataxin mRNA levels following transfection (Figure 5). The gene discussed is SRF; the disease is Friedreich ataxia.