Assessment of the expression levels of survivin, p53, and pERK1-2 proteins by immunohistochemistry (IHC) as well as characterization of the chromosomal copy number of cyclinD1 and h-prune genes by fluorescence in situ hybridization (FISH) analysis was carried out on paraffin-embedded tissue sections from primary breast carcinomas of our series. The gene discussed is PRUNE1; the disease is breast carcinoma.