To assess tumor growth after Msi1 KD, spheroid cultures of T47D and MCF-7 cells expressing either the non-silencing or Msi1 shRNA were engrafted into nude mice at either 10,000 cells/site (T47D) or 100,000 cells/site (MCF-7) (Figure 5A). This evidence concerns the gene MSI1 and neoplasm.