Western blot analysis of phospho-STAT-1(Tyr701) (active form) levels, normalised against p85/p91 STAT-1 total protein levels, showed that the interferon system response was triggered only at high MOI values (1:100), whereas infection at MOI 1:1000 did not have any effect on STAT-1 phosphorylation (data not shown). The gene discussed is STAT1; the disease is infection.