While all of the five SCCHN cell lines showed NF-κB DNA-binding activity (Fig. 2d), the DNA binding was significantly higher in the highly metastatic cells lines (Tb, TL and OSC-4) compared to the cells with lower metastatic potential (TSCC and Tca8113). The gene discussed is NFKB1; the disease is head and neck squamous cell carcinoma.