Nramp1-sufficient F1 Foxp3GFP reporter hemizygous male mice derived by intercrossing 129SvJ males with Foxp3GFP/GFP females (on the C57BL/6 background) that simultaneously allow persistent Salmonella infection and for all Tregs to be isolated based on cell sorting for GFP+(Foxp3+) cells were used in these experiments [23] (Figure 5A). The gene discussed is FOXP3; the disease is Salmonella Infections.