CD8A and infection: Single-cell RT-PCR and sequencing of the CDR3β region of tetramer+Vβ8.3+CD8+ T cells following either HK or HK-NPN3A infections showed that (Table S1) the mutant DbNPN3A+CD8+ T cells utilized different Jβ regions (primary (10): Jβ1.1, Jβ1.3; secondary (20): Jβ1.1, Jβ1.6, Jβ2.2) in comparison to the wt DbNP366+CD8+ population (10: Jβ2.2, Jβ2.4; 20: Jβ 2.2), and showed evidence of more variable CDR3β loop lengths (8–9 aa).