SP100 and primary biliary cholangitis: Further testing for ANA-PBC-specific antibodies and AMA was done by molecularly based assays, namely, IgG-specific ELISAs (INOVA Diagnostics, San Diego, CA), which proved also positive for anti-sp100 (55.3 Units; UNL: 20 Units) and M2 IgG-isotype specific AMA (92 Units), respectively.