To further confirm that MiTF plays a key role in cell survival after UVC radiation, MiTF was knocked down in SK-Mel-28 melanoma cell line by 2 different shRNA constructs Mish1 and Mish2 (Fig 5E); cells were exposed to 2 and 4 mJ/cm2 of UVC, and colonies were counted 2 weeks later. The gene discussed is MITF; the disease is melanoma.