In this research, we found that non-CpG ODN(ODN2006 control) can also increase the invasion of glioma cells through TLR9 signaling pathway, which indicated that non-CpG ODNs (ODNs from in vivo cells such as dying tumor cells) may also be able to increase the invasion of glioma through TLR9 pathway. The gene discussed is TLR9; the disease is central nervous system cancer.