The aqueous level of VEGF was significantly elevated in the samples from Group D (patients with PDR) (318.6 ± 238.6 pg/ml [83.0 - 836.1] as well as in samples from Group C (patients with NPDR) (133.3 ± 58.5 pg/ml [43.4 – 279]) when compared with those from Group B patients (diabetic without retinopathy) (56.5 ± 27.5 pg/ml [28.3 – 114.2]) and Group A patients (non-diabetic control patients) (62.6 ± 48.8 pg/ml [8.4-228]); whereas, there was no significant difference between the aqueous levels of VEGF between Group A and Group B (P = 0.985). The gene discussed is VEGFA; the disease is retinal disorder.