To do so, semi-confluent 10T1/2 MSC were incubated with 10T1/2 media (RM), or different tumor cell (MIA-PaCa-2, MCF-7 and MDA-MB-231)-derived conditioned media (TCM) for 24 and 72 h and fixed and processed for immunofluroscent staining with alpha smooth muscle actin (α-SMA), a positive marker of mural cells [11]. Here, ACTA1 is linked to neoplasm.