With the purpose to analyze the effect of CA-074 on the metastatic potential of melanoma cells, mice were injected i.v. with MM4 cells, a very aggressive human melanoma cell line [26], being MM1 cells unable to induce artificial lung metastases when injected i.v. Interestingly, MM4 cells produced and secreted more than 50-fold VEGF-A (226.6 ± 31.3 pg/ml) when compared to MM1 cells (4.73 ± 1.9 pg/ml), a feature likely associated with the high aggressiveness shown by the MM4 cell line. This evidence concerns the gene VEGFA and melanoma.