In this study, in order to investigate the predictive value of the methylation status of a panel of TSGs on tumor recurrence in HCC, the promoter methylation of twelve TSGs that belonging to the molecular pathways involved in cell immortalization and transformation included P16, CDH1, GSTP1, DAPK, MGMT, XAF1, TIMP3, SOCS1, SFRP1, TMS1, SYK and DKK1 were initially examined in a small cohort of 20 cases of HCC treated with LT [12,22-31] (Table 1). This evidence concerns the gene TIMP3 and hepatocellular carcinoma.