In conclusion, we have demonstrated that FFA stimulates a different Ca2+ activation pattern in ciPod, which suggests that TRPC6 may be functionally different between human glomerular endothelial cells and podocytes through nephrin regulation, which in light of recent findings may play a role in the progression of glomerulosclerosis such as in FSGS. This evidence concerns the gene NPHS1 and glomerulosclerosis.