The results presented here show that when using the cancer cell-selective TETP promoter previously described for our melanoma/melanocyte-specific CRAd vector [40] in combination with two novel RD vectors expressing IL-2 and CD40L from a bicistronic expression cassette, only moderate 3-fold enhancements of IL-2 or CD40L were obtained, whereas controls including the CMV promoter allowed much stronger expression enhancement in the Ad vector co-replication system. Here, CD40LG is linked to melanoma.