When replacing the transgene vector AdCMV-eGFP with AdCMV-CD40L or AdCMV-IL-2, 49- and 131-fold trans-complementation-mediated transgene expression enhancement was found in melanoma M000301 cells, when combined with wtAd5, and 53- and 288-fold enhancement when combined with AdΔEP-TETP. The gene discussed is IL2; the disease is melanoma.