Comparison of the routinely assessed immunohistochemical HER2 status and FISH revealed that two invasive carcinomas diagnosed immunohistochemically as highly overexpressed (score 3+) did not show amplification of the HER2 gene by FISH, with the conclusion that these are false positives (Table 2; cases 2 and 3; Figure 1B). This evidence concerns the gene ERBB2 and invasive carcinoma.