Chronic hyperglycemia did not increased expression of several components of the NAD(P)H oxidase system and this was observed at both the protein and mRNA levels These results are similar to the Zucker fa/fa/rat (type 2) but differ from that observed by Bitnar et.al who observed increases in p47phox in younger GK rats [8], [51], [52]. This evidence concerns the gene NCF1 and Hyperglycemia.