To test whether the role of KRIT1 in controlling FoxO1 and SOD2 expression was supported by experimental procedures alternative and complementary to the genetic knockout technology, as well as confirmed in cell types more relevant to the CCM disease, we used the RNAi technology to knockdown KRIT1 expression in Human Umbilical Vein Endothelial Cells (HUVEC). The gene discussed is FOXO1; the disease is cerebral cavernous malformation.