Similar results were obtained whenimmunoprecipitations were performed using different antibodies recognizingdifferent epitopes on the bcl-2 and HIF-1α proteins (data not shown).Immunoprecipitation experiments of HIF-1α protein were also perfomed intwo other melanoma cell lines, JR8 and PLF2, and their bcl-2 derivative stablyclones treated with MG132 obtaining similar results (Figure 3C,D) and thus generalizing the ability of bcl-2 protein to interact withHIF-1α protein. This evidence concerns the gene GDI1 and melanoma.