Since previous studies have shown that NS1-mediated PI3K activation is obviously important for the efficient propagation of influenza A virus [7,14,18,20,21], in this study, we examined the effect of heterologous SH3 (h-SH3) on (i) the replicability of five strains of influenza A virus from three subtypes (H1N1, H3N2, and H9N2) in infected cells and (ii) the phosphorylation status of Akt after viral infection. The gene discussed is AKT1; the disease is viral infectious disease.