This current study provides no conclusive evidence, but contains some intriguing observations that, following ex vivo contact with M. tuberculosis specific antigens, recently acquainted infection with M. tuberculosis is related to a positive IFN-γ ELISpot and a negative IL-2 ELISpot while remote infection with M. tuberculosis is related to a positive IFN-γ ELISpot and a positive IL-2 ELISpot. This evidence concerns the gene IFNG and infection.