In the GBM8 tumor stem cell line, similar 80% inhibition of TWIST1 message (data not shown) resulted in a marked decrease in the number of wells that formed tumor spheres when plated at clonal dilution (30% vs. 67% in control GBM8 cells; p = 0.0033) and the mean number of spheres in each well containing spheres (1.0 vs 2.3 for control GBM8 cells; p = 0.002) (see Table 2). Here, TWIST1 is linked to neoplasm.