RASSF1 and metastatic malignant neoplasm in the lymph nodes: Mehrotra J et al. reported that lymph node metastasis exhibited a somewhat higher prevalence of methylation compared with the primary breast carcinoma for five genes (Cyclin D2, RAR-beta, Twist, RASSF1A, and HIN-1) with a statistically significant increase in methylation of HIN-1 (p = 0.04).