We next performed an initial PCR profiling with the remaining 8 AES-I PCR primer sets on a collection of 9 AES-I strains (including driver strain), 9 non-AES-I CF isolates, 7 non-CF clinical isolates, 6 environmental isolates and the common laboratory strains PAO1, PA103, PAK and ATCC 27853 (Table 2). This evidence concerns the gene TLE5 and cystic fibrosis.