To investigate intrarenal disease associated regulation of NRN1, ROBO1 and ROBO2 mRNA, we assessed the renal expression on microdissected glomeruli of cohorts with diabetic nephropathy (DN), nephrosclerosis (NSC), focal-segmental glomerulosclerosis (FSGS), membranous glomerulonephropathy (MGN) and pretransplant biopsies. The gene discussed is NRN1; the disease is focal segmental glomerulosclerosis.