S100B and obesity due to melanocortin 4 receptor deficiency: Accordingly, follow-up single group comparisons of BMI groups showed that S100B levels in obesity were significantly higher than in overweight (P = .006, Cohen's d = 2.25), and normal weight subjects (P = .001, Cohen's d = 2.90), or in overweight higher than in the latter (P = .049, Cohen's d = 0.65) (Figure 1(b)).