Furthermore, the activity of caspase-3 in cell lysates was increased as a consequence of TS depletion (Figure 4C), and previous exposure of lung cancer cells to the pan-caspase inhibitor ZVAD-FMK significantly inhibited the increase in the size of the sub-G1 cell population induced by depletion of TS (Figure 4D). Here, CASP3 is linked to lung cancer.