To determine whether PTEN and subsequent perturbation of the PI3K/AKT signaling pathway would play a significant role in sulforaphane-mediated effects on prostate carcinogenesis, we initially studied the human prostate cancer cell line PC3, which has a PTEN deletion, and found that it is more sensitive to growth inhibition by SF than the PNT1A cell line that has wild type PTEN expression. Here, PTEN is linked to prostate cancer.