In conclusion, this evaluation has shown that NS1 antigen capture assays are indeed an alternative tool for the early diagnosis of dengue infections, may be used as a screening test prior virus isolation and used in combination with IgM capture can increase the rate of cases confirmation, especially in endemic areas where secondary infections are expected to occur due to the co-circulation of the different DENV serotypes, such as seen in Brazil. Here, CD40LG is linked to infection.