Hepa 1-6 (mouse hepatoma), THLE-5B (non-tumorigenic human liver), HepG2 (human hepatoblastoma) and SNU-182 (human hepatoma) cell lines were treated with 4 mM CCl4 for 24 hours, and CD133 mRNA expression was determined. This evidence concerns the gene PROM1 and hepatocellular carcinoma.