As shown in Fig. 2D, by immunohistochemical analysis, the membrane staining of the epithelial marker E-cadherin significantly increased in the pulmonary metastatic nodules compared to the primary SPC-A-1sci tumour, and otherwise the mesenchymal marker, vimentin, remarkably decreased in the pulmonary metastatic nodules. The gene discussed is CDH1; the disease is neoplasm.