Here we have investigated the involvement of the clathrin pathway in BTV-1 entry and infection of BHK cells using deletion mutants of AP180 (AP180C) and Eps15 (Eps15-Ed95/295; from here known as DN-Esp15) which act as DN inhibitors of CME and block uptake of transferrin [5], [6], [51]. Here, EPS15 is linked to infection.