To addressthe in vivo relevance of the NF-κB suppression by SIRT1, weexamined the expression of two known NF-κB-dependent genes, ICAM-1 andVCAM-1, in aortae from young 8-week-old ApoE-/-SIRT1+/- and ApoE-/- SIRT1+/+ micewithout atherosclerosis in descending thoracic aortae 3 hours afterintraperitoneal injection of LPS. This evidence concerns the gene APOE and atherosclerosis.