Regarding chemokine release, PI3K inhibitor LY294002 and JNK inhibitor SP600125 could suppress IL-27 induced CCL2, CXCL9 and CXCL10 from control FLS, JAK inhibitor AG490, PI3K inhibitor LY294002 and JNK inhibitor SP600125 could suppress IL-27 induced CCL2 and CXCL10 release from RA-FLS, and JAK inhibitor AG490 and PI3K inhibitor LY294002 could inhibit IL-27 induced CXCL9 release from RA-FLS (Figure 7). The gene discussed is CXCL9; the disease is rheumatoid arthritis.